› 5g30

X-ray diffraction
1.65Å resolution

Crystallographic structure of mutant D60S of thioredoxin from Litopenaeus vannamei

Released:
DOI: 10.2210/pdb5g30/pdb
PDB entry 5g30 coloured by chain, front view.
PDB entry 5g30 coloured by chain, side view.
PDB entry 5g30 coloured by chain, top view.
Source organism: Penaeus vannamei
Primary publication:
Is dimerization a common feature in thioredoxins? The case of thioredoxin from Litopenaeus vannamei.
Campos-Acevedo AA, Sotelo-Mundo RR, Pérez J, Rudiño-Piñera E
Acta Crystallogr D Struct Biol 73 326-339 (2017)
PMID: 28375144

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109402 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 105 amino acids
Theoretical weight: 11.93 KDa
Source organism: Penaeus vannamei
Expression system: Escherichia coli
UniProt:
  • Canonical: B1PWB9 (Residues: 1-105; Coverage: 100%)
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand ACT 3 x ACT
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 58.491Å b: 82.712Å c: 82.206Å
α: 90° β: 102.58° γ: 90°
R-values:
R R work R free
0.167 0.165 0.201
Expression system: Escherichia coli

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Citations

7 citation in other articles

Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions.
Yang et al. (2017)
6 more