› 5fne

X-ray diffraction
1.5Å resolution

CRYSTAL STRUCTURE OF FUNGAL VERSATILE PEROXIDASE FROM PLEUROTUS ERYNGII TRIPLE MUTANT E37K, H39R & G330R

Released:
DOI: 10.2210/pdb5fne/pdb
PDB entry 5fne coloured by chain, front view.
PDB entry 5fne coloured by chain, side view.
PDB entry 5fne coloured by chain, top view.
Source organism: Pleurotus eryngii
Primary publication:
Unveiling the basis of alkaline stability of an evolved versatile peroxidase.
Sáez-Jiménez V, Acebes S, Garcia-Ruiz E, Romero A, Guallar V, Alcalde M, Medrano FJ, Martínez AT, Ruiz-Dueñas FJ
Biochem J 473 1917-28 (2016)
PMID: 27118867

Function and Biology Details

Reaction catalysed: 
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H(2)O(2) = 4-hydroxy-3-methoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Versatile peroxidase VPL2 Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 34.72 KDa
Source organism: Pleurotus eryngii
Expression system: Escherichia coli str. K-12 substr. W3110
UniProt:
  • Canonical: O94753 (Residues: 31-361; Coverage: 98%)
Gene name: vpl2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21212
Unit cell:
a: 55.19Å b: 104.2Å c: 76.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.229
Expression system: Escherichia coli str. K-12 substr. W3110

Quick links

Citations

4 citation in other articles

Mechanistic Insights into Dye-Decolorizing Peroxidase Revealed by Solvent Isotope and Viscosity Effects.
Shrestha et al. (2017)
3 more