Structure analysis

Mammalian 40S HCV-IRES complex

Electron Microscopy
3.9Å resolution
Source organism: Oryctolagus cuniculus
Assembly composition:
hetero 35-mer (preferred)
Entry contents: 33 distinct polypeptide molecules
2 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 35-mer
Accessible surface area: 455926.43 Å2
Buried surface area: 202289.58 Å2
Dissociation area: 1,564.95 Å2
Dissociation energy (ΔGdiss): 2.62 kcal/mol
Dissociation entropy (TΔSdiss): 14.84 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-140329

Macromolecules

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Chain: B
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P61247 (Residues: 1-264; Coverage: 100%)
Gene names: FTE1, MFTL, RPS3A
Pfam: Ribosomal S3Ae family
InterPro:

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Chain: H
Length: 194 amino acids
Theoretical weight: 22.17 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62081 (Residues: 1-194; Coverage: 100%)
Gene name: RPS7
Pfam: Ribosomal protein S7e
InterPro:

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Chain: K
Length: 165 amino acids
Theoretical weight: 18.93 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P46783 (Residues: 1-165; Coverage: 100%)
Gene name: RPS10
Pfam: Plectin/S10 domain
InterPro:
CATH: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

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Chain: O
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62263 (Residues: 1-151; Coverage: 100%)
Gene names: PRO2640, RPS14
Pfam: Ribosomal protein S11
InterPro:
CATH: Ribosomal protein S11

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Chain: R
Length: 135 amino acids
Theoretical weight: 15.58 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P08708 (Residues: 1-135; Coverage: 100%)
Gene names: RPS17, RPS17L
Pfam: Ribosomal S17
InterPro:
CATH: Ribosomal protein S17e-like

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Chain: T
Length: 145 amino acids
Theoretical weight: 16.09 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P39019 (Residues: 1-145; Coverage: 100%)
Gene name: RPS19
Pfam: Ribosomal protein S19e
InterPro:

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Chain: V
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P63220 (Residues: 1-83; Coverage: 100%)
Gene name: RPS21
Pfam: Ribosomal protein S21e
InterPro:
CATH: Hypothetical Cytosolic Protein; Chain: A;

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Chain: W
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62244 (Residues: 1-130; Coverage: 100%)
Gene names: OK/SW-cl.82, RPS15A
Pfam: Ribosomal protein S8
InterPro:
CATH:

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Chain: X
Length: 143 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62266 (Residues: 1-143; Coverage: 100%)
Gene name: RPS23
Pfam: Ribosomal protein S12/S23
InterPro:

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Chain: Y
Length: 133 amino acids
Theoretical weight: 15.46 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62847 (Residues: 1-133; Coverage: 100%)
Gene name: RPS24
Pfam: Ribosomal protein S24e
InterPro:
CATH: Alpha-Beta Plaits

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Chain: Z
Length: 125 amino acids
Theoretical weight: 13.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62851 (Residues: 1-125; Coverage: 100%)
Gene name: RPS25
Pfam: S25 ribosomal protein
InterPro:
CATH: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

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Chain: a
Length: 115 amino acids
Theoretical weight: 13.05 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62854 (Residues: 1-115; Coverage: 100%)
Gene name: RPS26
Pfam: Ribosomal protein S26e
InterPro:
CATH: Ribosomal protein S26e

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Chain: b
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P42677 (Residues: 1-84; Coverage: 100%)
Gene names: MPS1, RPS27
Pfam: Ribosomal protein S27
InterPro:
CATH: Zn-binding ribosomal proteins

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Chain: c
Length: 69 amino acids
Theoretical weight: 7.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62857 (Residues: 1-69; Coverage: 100%)
Gene name: RPS28
Pfam: Ribosomal protein S28e
InterPro:
CATH: Nucleic acid-binding proteins

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Chain: e
Length: 59 amino acids
Theoretical weight: 6.67 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62861 (Residues: 75-133; Coverage: 44%)
Gene name: FAU
Pfam: Ribosomal protein S30
InterPro: Small ribosomal subunit protein eS30

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Name: 18S Ribosomal RNA
Representative chains: 1
Length: 1869 nucleotides
Theoretical weight: 602.78 KDa
Rfam: Eukaryotic small subunit ribosomal RNA

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Name: HCV-IRES
Representative chains: z
Length: 504 nucleotides
Theoretical weight: 162.19 KDa
Rfam:
  • Hepatitis C virus internal ribosome entry site
  • Hepatitis C alternative reading frame stem-loop
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