› 5ffn

X-ray diffraction
1.8Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103165 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidase S8/S53 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 31.81 KDa
Source organism: Bacillus sp. (in: firmicutes)
Expression system: Bacillus subtilis
UniProt:
  • Canonical: A0A182DWC8 (Residues: 1-311; Coverage: 100%)
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
Subtilisin-chymotrypsin inhibitor-2A Chain: I
Molecule details ›
Chain: I
Length: 72 amino acids
Theoretical weight: 8.11 KDa
Source organism: Hordeum vulgare
Expression system: Escherichia coli
UniProt:
  • Canonical: P01053 (Residues: 13-84; Coverage: 86%)
Sequence domains: Potato inhibitor I family
Structure domains: Trypsin Inhibitor V, subunit A

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 58.753Å b: 66.838Å c: 107.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.188
Expression systems:
  • Bacillus subtilis
  • Escherichia coli

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