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Crystal Structure of a PHD finger bound to histone H3 T3ph peptide

X-ray diffraction
2.702Å resolution
PDB entry 5fb0 coloured by chain, front view.
PDB entry 5fb0 coloured by chain, side view.
PDB entry 5fb0 coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Nuclear autoantigen Sp-100 and Histone H3.1
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Multimeric state: hetero dimer
Accessible surface area: 10918.13 Å2
Buried surface area: 1250.0 Å2
Dissociation area: 625 Å2
Dissociation energy (ΔGdiss): 4.69 kcal/mol
Dissociation entropy (TΔSdiss): 7.68 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-149986
Assembly 2
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Assembly Name: Nuclear autoantigen Sp-100 and Histone H3.1
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Multimeric state: hetero dimer
Accessible surface area: 10712.24 Å2
Buried surface area: 1246.95 Å2
Dissociation area: 623.47 Å2
Dissociation energy (ΔGdiss): 5.3 kcal/mol
Dissociation entropy (TΔSdiss): 7.67 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-149986

Macromolecules

Nuclear autoantigen Sp-100
Chains: A, C
Length: 184 amino acids
Theoretical weight: 21.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23497 (Residues: 466-498, 499-508; Coverage: 5%)
Gene name: SP100
InterPro:
CATH:
Nuclear autoantigen Sp-100 in PDB entry 5fb0, assembly 1, front view.
Nuclear autoantigen Sp-100 in PDB entry 5fb0, assembly 1, side view.
Nuclear autoantigen Sp-100 in PDB entry 5fb0, assembly 1, top view.
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Histone H3.1
Chains: D, F
Length: 15 amino acids
Theoretical weight: 1.65 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-16; Coverage: 11%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Histone H3.1 in PDB entry 5fb0, assembly 1, front view.
Histone H3.1 in PDB entry 5fb0, assembly 1, side view.
Histone H3.1 in PDB entry 5fb0, assembly 1, top view.
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