PDBe 5f4e

X-ray diffraction
2.4Å resolution

Crystal structure of the human sperm Izumo1 and egg Juno complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Izumo sperm-egg fusion protein 1 Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.14 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q8IYV9 (Residues: 22-254; Coverage: 71%)
  • Best match: Q8IYV9-2 (Residues: 22-169)
Gene name: IZUMO1
Sperm-egg fusion protein Juno Chain: B
Molecule details ›
Chain: B
Length: 215 amino acids
Theoretical weight: 24.58 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: A6ND01 (Residues: 20-228; Coverage: 91%)
Gene names: FOLR4, IZUMO1R, JUNO
Sequence domains: Folate receptor family

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 148Å b: 55.8Å c: 81.6Å
α: 90° β: 103.6° γ: 90°
R-values:
R R work R free
0.182 0.18 0.222
Expression system: Drosophila melanogaster