PDBe 5ewq

X-ray diffraction
2.57Å resolution

The crystal structure of an amidase family protein from Bacillus anthracis str. Ames

Released:
Source organism: Bacillus anthracis
Entry authors: Tan K, Zhou M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidase family protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 513 amino acids
Theoretical weight: 57.56 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0F7RKC8 (Residues: 27-536; Coverage: 95%)
Gene name: GBAA_1921
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 77.39Å b: 89.558Å c: 89.6Å
α: 112.03° β: 106.64° γ: 106.55°
R-values:
R R work R free
0.222 0.22 0.254
Expression system: Escherichia coli