› 5elf

X-ray diffraction
1.55Å resolution

Cholera toxin El Tor B-pentamer in complex with A-pentasaccharide

Released:
DOI: 10.2210/pdb5elf/pdb
PDB entry 5elf coloured by chain, front view.
PDB entry 5elf coloured by chain, side view.
PDB entry 5elf coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence.
Heggelund JE, Burschowsky D, Bjørnestad VA, Hodnik V, Anderluh G, Krengel U
PLoS Pathog 12 e1005567 (2016)
PMID: 27082955

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Cholera enterotoxin subunit B Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 103 amino acids
Theoretical weight: 11.66 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Vibrio
UniProt:
  • Canonical: P01556 (Residues: 22-124; Coverage: 100%)
Gene names: VC_1456, ctxB, toxB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: FUC, GLC, GAL, A2G
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FUC, GAL, A2G, BGC
Carbohydrate polymer
5 bound ligands:
Ligand CA 10 x CA
Ligand BCN 10 x BCN
Ligand FUC 2 x FUC
Ligand GLC 2 x GLC
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 63.626Å b: 84.72Å c: 195.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.22
Expression system: Vibrio

Quick links

Citations

7 review citations

Histo-blood group antigens as mediators of infections.
Heggelund et al. (2017)
6 more

1 mention without citation

High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence.
Heggelund et al. (2016)