› 5e24

X-ray diffraction
2.14Å resolution

Structure of the Su(H)-Hairless-DNA Repressor Complex

Released:
DOI: 10.2210/pdb5e24/pdb
PDB entry 5e24 coloured by chain, front view.
PDB entry 5e24 coloured by chain, side view.
PDB entry 5e24 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and Function of the Su(H)-Hairless Repressor Complex, the Major Antagonist of Notch Signaling in Drosophila melanogaster.
Yuan Z, Praxenthaler H, Tabaja N, Torella R, Preiss A, Maier D, Kovall RA
PLoS Biol 14 e1002509 (2016)
PMID: 27404588

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer
hetero pentamer (preferred)
hetero octamer
PDBe Complex ID:
PDB-CPX-118603 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 370 amino acids
Theoretical weight: 40.45 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Protein hairless Chains: B, D
Molecule details ›
Chains: B, D
Length: 38 amino acids
Theoretical weight: 4.34 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02308 (Residues: 232-269; Coverage: 4%)
Gene names: CG5460, H
Suppressor of hairless protein Chains: E, F
Molecule details ›
Chains: E, F
Length: 424 amino acids
Theoretical weight: 48.04 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P28159 (Residues: 99-522; Coverage: 71%)
Gene names: CG3497, Su(H), dRBP-JK
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 15 nucleotides
Theoretical weight: 4.5 KDa
DNA (5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3') Chain: H
Molecule details ›
Chain: H
Length: 15 nucleotides
Theoretical weight: 4.67 KDa

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand EDO 113 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 177.71Å b: 93.89Å c: 154.39Å
α: 90° β: 109.83° γ: 90°
R-values:
R R work R free
0.174 0.173 0.193
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Notch Signaling in Development, Tissue Homeostasis, and Disease.
Siebel et al. (2017)
3 more

10 mentions without citation

Structurally conserved binding motifs of transcriptional regulators to notch nuclear effector CSL.
Hall et al. (2019)
9 more