› 5e13

X-ray diffraction
1.34Å resolution

Crystal structure of Eosinophil-derived neurotoxin in complex with the triazole double-headed ribonucleoside 11c

Released:
DOI: 10.2210/pdb5e13/pdb
PDB entry 5e13 coloured by chain, front view.
PDB entry 5e13 coloured by chain, side view.
PDB entry 5e13 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Triazole double-headed ribonucleosides as inhibitors of eosinophil derived neurotoxin.
Chatzileontiadou DS, Parmenopoulou V, Manta S, Kantsadi AL, Kylindri P, Griniezaki M, Kontopoulou F, Telopoulou A, Prokova H, Panagopoulos D, Boix E, Balatsos NA, Komiotis D, Leonidas DD
Bioorg Chem 63 152-65 (2015)
PMID: 26551065

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-secretory ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli B
UniProt:
  • Canonical: P10153 (Residues: 1-161; Coverage: 100%)
Gene names: EDN, RNASE2, RNS2
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand 5J9 1 x 5J9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P212121
Unit cell:
a: 42.02Å b: 52.18Å c: 56.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.198
Expression system: Escherichia coli B

Quick links

Citations

2 review citations

Interactions between polycyclic aromatic hydrocarbons and epoxide hydrolase 1 play roles in asthma.
Wang et al. (2019)
1 more

4 mentions without citation

Gene therapy for hemophilia.
Nathwani AC. (2022)
3 more