› 5do8

X-ray diffraction
1.8Å resolution

1.8 Angstrom crystal structure of Listeria monocytogenes Lmo0184 alpha-1,6-glucosidase

Released:
DOI: 10.2210/pdb5do8/pdb
PDB entry 5do8 coloured by chain, front view.
PDB entry 5do8 coloured by chain, side view.
PDB entry 5do8 coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
Structure to function of an α-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis.
Light SH, Cahoon LA, Halavaty AS, Freitag NE, Anderson WF
Nat Microbiol 2 16202 (2016)
PMID: 27819654

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187026 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
oligo-1,6-glucosidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 555 amino acids
Theoretical weight: 65.34 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YAE6 (Residues: 1-553; Coverage: 100%)
Gene name: lmo0184
Sequence domains: Alpha amylase, catalytic domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand BGC 3 x BGC
Ligand CL 4 x CL
Ligand BTB 3 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 88.123Å b: 103.937Å c: 193.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.188
Expression system: Escherichia coli

Quick links

Citations

17 citation in other articles

A flavin-based extracellular electron transfer mechanism in diverse Gram-positive bacteria.
Light et al. (2018)
16 more

3 mentions without citation

New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag.
Biancucci et al. (2017)
2 more