› 5dhd

X-ray diffraction
1.27Å resolution

Crystal structure of ChBD2 from Thermococcus kodakarensis KOD1

Released:
DOI: 10.2210/pdb5dhd/pdb
PDB entry 5dhd coloured by chain, front view.
PDB entry 5dhd coloured by chain, side view.
PDB entry 5dhd coloured by chain, top view.
Source organism: Thermococcus kodakarensis KOD1
Primary publication:
Crystal structures of chitin binding domains of chitinase from Thermococcus kodakarensis KOD1.
Hanazono Y, Takeda K, Niwa S, Hibi M, Takahashi N, Kanai T, Atomi H, Miki K
FEBS Lett 590 298-304 (2016)
PMID: 26823175

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitinase Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 10.93 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UWR7 (Residues: 621-720; Coverage: 8%)
Gene names: Pk-chiA, TK1765
Sequence domains: Cellulose binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand PE3 6 x PE3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P65
Unit cell:
a: 60.245Å b: 60.245Å c: 68.845Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.195 0.195
Expression system: Escherichia coli

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Citations

3 review citations

An overview of 25 years of research on Thermococcus kodakarensis, a genetically versatile model organism for archaeal research.
Rashid et al. (2020)
2 more