› 5dbk

X-ray diffraction
3.24Å resolution

apo form of the quorum sensor NprR from B. thuringiensis

Released:
DOI: 10.2210/pdb5dbk/pdb
PDB entry 5dbk coloured by chain, front view.
PDB entry 5dbk coloured by chain, side view.
PDB entry 5dbk coloured by chain, top view.
Source organism: Bacillus thuringiensis Bt407
Primary publication:
How Quorum Sensing Connects Sporulation to Necrotrophism in Bacillus thuringiensis.
Perchat S, Talagas A, Poncet S, Lazar N, Li de la Sierra-Gallay I, Gohar M, Lereclus D, Nessler S
PLoS Pathog 12 e1005779 (2016)
PMID: 27483473

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 44.36 KDa
Source organism: Bacillus thuringiensis Bt407
Expression system: Escherichia coli
UniProt:
  • Canonical: M1Q3P9 (Residues: 60-423; Coverage: 86%)
Gene name: H175_ch0523
Sequence domains: Tetratricopeptide repeat
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 67.789Å b: 105.337Å c: 177.068Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.245
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Genetic and Structural Analyses of RRNPP Intercellular Peptide Signaling of Gram-Positive Bacteria.
Neiditch et al. (2017)
4 more

3 mentions without citation

How Quorum Sensing Connects Sporulation to Necrotrophism in Bacillus thuringiensis.
Perchat et al. (2016)
2 more