PDBe 5cxx

X-ray diffraction
1.55Å resolution

Structure of a CE1 ferulic acid esterase, AmCE1/Fae1A, from Anaeromyces mucronatus in complex with Ferulic acid

Released:

Function and Biology Details

Reaction catalysed:
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fae1A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 275 amino acids
Theoretical weight: 31.08 KDa
Source organism: Anaeromyces mucronatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F2YCB6 (Residues: 1-275; Coverage: 100%)
Gene name: fae1A
Sequence domains: Putative esterase
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: I222
Unit cell:
a: 99.79Å b: 152.65Å c: 154.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.141 0.16
Expression system: Escherichia coli BL21(DE3)