5cir

X-ray diffraction
3Å resolution

Crystal structure of death receptor 4 (DR4; TNFFRSF10A) bound to TRAIL (TNFSF10)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-126166 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor ligand superfamily member 10 Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 169 amino acids
Theoretical weight: 19.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50591 (Residues: 113-281; Coverage: 60%)
Gene names: APO2L, TNFSF10, TRAIL
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
Tumor necrosis factor receptor superfamily member 10A Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 108 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00220 (Residues: 125-232; Coverage: 24%)
Gene names: APO2, DR4, TNFRSF10A, TRAILR1
Sequence domains: TNFR/NGFR cysteine-rich region
Structure domains: Tumor Necrosis Factor Receptor, subunit A, domain 2

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 84.6Å b: 87.6Å c: 107.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.216
Expression system: Escherichia coli BL21(DE3)