Function and Biology Details
Reaction catalysed:
L-glutamate 1-semialdehyde + NADP(+) + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Glutamyl-tRNA reductase
- Tetratricopeptide-like helical domain superfamily
- Glutamyl-tRNA reductase, N-terminal domain superfamily
- Protein FLUORESCENT IN BLUE LIGHT
- Domain of unknown function DUF2470
- Haem oxygenase HugZ-like superfamily
- FMN-binding split barrel
- Tetrapyrrole biosynthesis, glutamyl-tRNA reductase, dimerisation domain
5 more domains
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-154888 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glutamyl-tRNA reductase 1, chloroplastic
Molecule details ›
Chains: A, B
Length: 472 amino acids
Theoretical weight: 52.14 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 472 amino acids
Theoretical weight: 52.14 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
- Canonical:
P42804 (Residues: 73-543; Coverage: 87%)
Sequence domains:
- Glutamyl-tRNAGlu reductase, N-terminal domain
- Shikimate / quinate 5-dehydrogenase
- Glutamyl-tRNAGlu reductase, dimerisation domain
Glutamyl-tRNA reductase-binding protein, chloroplastic
Molecule details ›
Chains: C, D
Length: 310 amino acids
Theoretical weight: 34.27 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
Sequence domains: Domain of unknown function (DUF2470)
Structure domains:
Length: 310 amino acids
Theoretical weight: 34.27 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
- Canonical: Q9LU39 (Residues: 42-317; Coverage: 87%)
Sequence domains: Domain of unknown function (DUF2470)
Structure domains:
Protein FLUORESCENT IN BLUE LIGHT, chloroplastic
Molecule details ›
Chains: E, F
Length: 159 amino acids
Theoretical weight: 17.77 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
Sequence domains: Tetratricopeptide repeat
Structure domains: Tetratricopeptide repeat domain
Length: 159 amino acids
Theoretical weight: 17.77 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
- Canonical: Q940U6 (Residues: 195-316; Coverage: 39%)
Sequence domains: Tetratricopeptide repeat
Structure domains: Tetratricopeptide repeat domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRF BEAMLINE BL17U
Spacegroup:
C2
Expression system: Escherichia coli
