› 5c92

X-ray diffraction
2.1Å resolution

Novel fungal alcohol oxidase with catalytic diversity among the AA5 family, in complex with copper

Released:
DOI: 10.2210/pdb5c92/pdb
PDB entry 5c92 coloured by chain, front view.
PDB entry 5c92 coloured by chain, side view.
PDB entry 5c92 coloured by chain, top view.
Source organism: Colletotrichum graminicola M1.001
Primary publication:
Structure-function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family.
Yin DT, Urresti S, Lafond M, Johnston EM, Derikvand F, Ciano L, Berrin JG, Henrissat B, Walton PH, Davies GJ, Brumer H
Nat Commun 6 10197 (2015)
PMID: 26680532

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose oxidase-like Early set domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 52.39 KDa
Source organism: Colletotrichum graminicola M1.001
Expression system: Komagataella pastoris
UniProt:
  • Canonical: E3QHV8 (Residues: 25-506; Coverage: 100%)
Gene name: GLRG_05590
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NAG 2 x NAG
Ligand CU1 1 x CU1
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 81.58Å b: 81.58Å c: 154.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.142 0.201
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Lignin degradation: microorganisms, enzymes involved, genomes analysis and evolution.
Janusz et al. (2017)
4 more

2 mentions without citation

Structure-function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family.
Yin et al. (2015)
1 more