› 5bn5

X-ray diffraction
3Å resolution

Structural basis for a unique ATP synthase core complex from Nanoarcheaum equitans

Released:
DOI: 10.2210/pdb5bn5/pdb
PDB entry 5bn5 coloured by chain, front view.
PDB entry 5bn5 coloured by chain, side view.
PDB entry 5bn5 coloured by chain, top view.
Source organism: Nanoarchaeum equitans Kin4-M
Primary publication:
Structural Basis for a Unique ATP Synthase Core Complex from Nanoarcheaum equitans.
Mohanty S, Jobichen C, Chichili VPR, Velázquez-Campoy A, Low BC, Hogue CWV, Sivaraman J
J Biol Chem 290 27280-27296 (2015)
PMID: 26370083

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181078 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
V-type ATP synthase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 570 amino acids
Theoretical weight: 64.77 KDa
Source organism: Nanoarchaeum equitans Kin4-M
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q74MJ7 (Residues: 1-570; Coverage: 100%)
Gene names: NEQ103, atpA
Sequence domains:
Structure domains:
V-type ATP synthase beta chain Chain: B
Molecule details ›
Chain: B
Length: 416 amino acids
Theoretical weight: 46.67 KDa
Source organism: Nanoarchaeum equitans Kin4-M
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q74MS5 (Residues: 1-416; Coverage: 100%)
Gene name: NEQ263
Sequence domains: ATP synthase alpha/beta family, nucleotide-binding domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: R3
Unit cell:
a: 192.649Å b: 192.649Å c: 108.566Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.221 0.254
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Genomic diversity, lifestyles and evolutionary origins of DPANN archaea.
Dombrowski et al. (2019)
8 other articles

1 mention without citation

Structural Basis for a Unique ATP Synthase Core Complex from Nanoarcheaum equitans.
Mohanty et al. (2015)