› 5arl

X-ray diffraction
2.61Å resolution

crystal structure of porcine RNase 4 D80A mutant in complex with dCMP

Released:
DOI: 10.2210/pdb5arl/pdb
PDB entry 5arl coloured by chain, front view.
PDB entry 5arl coloured by chain, side view.
PDB entry 5arl coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural basis of substrate specificity in porcine RNase 4.
Liang S, Acharya KR
FEBS J 283 912-28 (2016)
PMID: 26748441

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 134 amino acids
Theoretical weight: 15.66 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P15468 (Residues: 24-147; Coverage: 96%)
Gene names: RNASE4, RNS4
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand DCM 4 x DCM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P1
Unit cell:
a: 30.962Å b: 62.515Å c: 70.216Å
α: 89.78° β: 79.21° γ: 78.9°
R-values:
R R work R free
0.255 0.252 0.3
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Immune Modulation by Human Secreted RNases at the Extracellular Space.
Lu et al. (2018)
3 more

1 mention without citation

Structural basis of substrate specificity in porcine RNase 4.
Liang et al. (2016)