› 5ap9

X-ray diffraction
1.8Å resolution

Controlled lid-opening in Thermomyces lanuginosus lipase - a switch for activity and binding

Released:
DOI: 10.2210/pdb5ap9/pdb
PDB entry 5ap9 coloured by chain, front view.
PDB entry 5ap9 coloured by chain, side view.
PDB entry 5ap9 coloured by chain, top view.
Source organism: Thermomyces lanuginosus
Primary publication:
Controlled lid-opening in Thermomyces lanuginosus lipase- An engineered switch for studying lipase function.
Skjold-Jørgensen J, Vind J, Moroz OV, Blagova E, Bhatia VK, Svendsen A, Wilson KS, Bjerrum MJ
Biochim Biophys Acta Proteins Proteom 1865 20-27 (2017)
PMID: 27693248

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129890 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.32 KDa
Source organism: Thermomyces lanuginosus
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: O59952 (Residues: 23-291; Coverage: 98%)
Gene name: LIP
Sequence domains:
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand NAG 1 x NAG
Ligand GOL 2 x GOL
Ligand PEG 1 x PEG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P43212
Unit cell:
a: 90.478Å b: 90.478Å c: 160.455Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.197
Expression system: Aspergillus oryzae

Quick links

Citations

2 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
1 more

2 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
1 more