PDBe 5yoy

X-ray diffraction
2.73Å resolution

Crystal structure of the human tumor necrosis factor in complex with golimumab Fv

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tumor necrosis factor, soluble form Chains: A, B, C, J, K, L
Molecule details ›
Chains: A, B, C, J, K, L
Length: 173 amino acids
Theoretical weight: 19.35 KDa
Source organism: Homo sapiens
Expression system: Brevibacillus choshinensis
UniProt:
  • Canonical: P01375 (Residues: 76-233; Coverage: 68%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Golimumab light chain variable region Chains: D, E, F, M, N, O
Molecule details ›
Chains: D, E, F, M, N, O
Length: 119 amino acids
Theoretical weight: 13.02 KDa
Source organism: Homo sapiens
Expression system: Brevibacillus choshinensis
Golimumab heavy chain variable region Chains: G, H, I, P, Q, R
Molecule details ›
Chains: G, H, I, P, Q, R
Length: 129 amino acids
Theoretical weight: 14.18 KDa
Source organism: Homo sapiens
Expression system: Brevibacillus choshinensis

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 133.64Å b: 85.3Å c: 135.96Å
α: 90° β: 101.61° γ: 90°
R-values:
R R work R free
0.192 0.19 0.234
Expression system: Brevibacillus choshinensis