PDBe 5y25

X-ray diffraction
3.1Å resolution

EGFR kinase domain mutant (T790M/L858R) with covalent ligand NS-062

Released:
Source organism: Homo sapiens
Entry authors: Shiroishi M, Abe Y, Caaveiro JMM, Sakamoto S, Morimoto S, Fuchida H, Shindo N, Ojida A

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 38.15 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 698-1022; Coverage: 27%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine kinase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: I23
Unit cell:
a: 148.291Å b: 148.291Å c: 148.291Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.213 0.246
Expression system: Spodoptera frugiperda