PDBe 5xte

Electron Microscopy
3.4Å resolution

Cryo-EM structure of human respiratory complex III (cytochrome bc1 complex)

Released:
Source organism: Homo sapiens
Primary publication:
Architecture of Human Mitochondrial Respiratory Megacomplex I2III2IV2.
Cell 170 1247-1257.e12 (2017)
PMID: 28844695
Related structures: EMD-6774

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
Cytochrome b-c1 complex subunit 8 Chains: A, N
Molecule details ›
Chains: A, N
Length: 81 amino acids
Theoretical weight: 9.79 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14949 (Residues: 2-82; Coverage: 99%)
Gene name: UQCRQ
Sequence domains: UcrQ family
Cytochrome b-c1 complex subunit 9 Chains: B, O
Molecule details ›
Chains: B, O
Length: 57 amino acids
Theoretical weight: 5.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P47985 (Residues: 1-57; Coverage: 21%)
Gene name: UQCRFS1
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: C, P
Molecule details ›
Chains: C, P
Length: 196 amino acids
Theoretical weight: 21.65 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P47985 (Residues: 79-274; Coverage: 72%)
Gene name: UQCRFS1
Sequence domains:
Cytochrome b-c1 complex subunit 9 Chains: D, Q
Molecule details ›
Chains: D, Q
Length: 62 amino acids
Theoretical weight: 7.19 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UDW1 (Residues: 2-63; Coverage: 98%)
Gene names: HSPC119, UCRC, UQCR10
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: E, R
Molecule details ›
Chains: E, R
Length: 75 amino acids
Theoretical weight: 8.99 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07919 (Residues: 17-91; Coverage: 82%)
Gene name: UQCRH
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Cytochrome b-c1 complex subunit 7 Chains: F, S
Molecule details ›
Chains: F, S
Length: 106 amino acids
Theoretical weight: 13.04 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P14927 (Residues: 6-111; Coverage: 96%)
Gene names: UQBP, UQCRB
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Cytochrome b-c1 complex subunit 10 Chains: G, T
Molecule details ›
Chains: G, T
Length: 51 amino acids
Theoretical weight: 5.96 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14957 (Residues: 2-52; Coverage: 91%)
Gene names: UQCR, UQCR11
Cytochrome c1, heme protein, mitochondrial Chains: H, U
Molecule details ›
Chains: H, U
Length: 241 amino acids
Theoretical weight: 27.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P08574 (Residues: 85-325; Coverage: 74%)
Gene name: CYC1
Sequence domains: Cytochrome C1 family
Cytochrome b Chains: J, V
Molecule details ›
Chains: J, V
Length: 378 amino acids
Theoretical weight: 42.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00156 (Residues: 2-379; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: K, W
Molecule details ›
Chains: K, W
Length: 419 amino acids
Theoretical weight: 44.95 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P22695 (Residues: 35-453; Coverage: 93%)
Gene name: UQCRC2
Sequence domains:
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 446 amino acids
Theoretical weight: 49.18 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P31930 (Residues: 35-480; Coverage: 93%)
Gene name: UQCRC1
Sequence domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM

Cofactor: Ligand HEC 2 x HEC

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-6774