PDBe 5xfq

X-ray diffraction
2.4Å resolution

Ternary complex of PHF1, a DNA duplex and a histone peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
PHD finger protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 37.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q9Z1B8 (Residues: 25-360; Coverage: 60%)
Gene names: Phf1, Plc1, Tctex-3, Tctex3
Sequence domains:
Histone H3.1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 13 amino acids
Theoretical weight: 1.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P68431 (Residues: 30-42; Coverage: 10%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
DNA (5'-D(*GP*GP*GP*CP*GP*GP*CP*CP*GP*CP*CP*CP*T)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 13 nucleotides
Theoretical weight: 3.97 KDa

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 141.243Å b: 62.55Å c: 97.338Å
α: 90° β: 103.04° γ: 90°
R-values:
R R work R free
0.224 0.222 0.258
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided