PDBe 5wzz

X-ray diffraction
2.1Å resolution

The SIAH E3 ubiquitin ligases promote Wnt/ beta-catenin signaling through mediating Wnt-induced Axin degradation

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase SIAH1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 21.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: NEW Q8IUQ4 (Residues: 93-282; Coverage: 67%)
Gene names: HUMSIAH, SIAH1
Axin-1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 20 amino acids
Theoretical weight: 2.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: NEW O15169 (Residues: 375-394; Coverage: 2%)
Gene names: AXIN, AXIN1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 41.502Å b: 86.45Å c: 117.454Å
α: 90° β: 98.43° γ: 90°
R-values:
R R work R free
0.186 0.184 0.208
Expression system: Escherichia coli K-12