PDBe 5wxf

X-ray diffraction
1.46Å resolution

Crystal structure of uPA in complex with upain-2-2


Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 253 amino acids
Theoretical weight: 28.44 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
  • Canonical: NEW P00749 (Residues: 179-431; Coverage: 62%)
Gene name: PLAU
Sequence domains: Trypsin
upain-2-2 peptide Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.36 KDa
Source organism: Phage display vector pTDisp
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 115.137Å b: 115.137Å c: 41.307Å
α: 90° β: 90° γ: 120°
R R work R free
0.211 0.209 0.245
Expression systems:
  • Komagataella pastoris
  • Not provided