PDBe 5wmn

X-ray diffraction
1.82Å resolution

Crystal Structure of HLA-B7 in complex with SPI, an influenza peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, B-7 alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 276 amino acids
Theoretical weight: 32.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
SPI peptide from Influenza A virus Chains: E, F
Molecule details ›
Chains: E, F
Length: 9 amino acids
Theoretical weight: 992 Da
Source organism: unidentified influenza virus
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 69.396Å b: 84.262Å c: 77.945Å
α: 90° β: 104° γ: 90°
R-values:
R R work R free
0.186 0.184 0.222
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided