PDBe 5wle

X-ray diffraction
1.95Å resolution

Crystal structure of the PPS PHD finger in complex with H3K4me3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein partner of snf, isoform A Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 7.32 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q9VG78 (Residues: 904-965; Coverage: 3%)
Gene names: CG6525, Dmel\CG6525, Dmel_CG6525, FBgn0082831, PPS, SPP, Spp, pps, spp
Sequence domains: PHD-finger
Histone H3.1 Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P68431 (Residues: 2-13; Coverage: 9%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 22.972Å b: 44.223Å c: 31.308Å
α: 90° β: 94.77° γ: 90°
R-values:
R R work R free
0.149 0.145 0.186
Expression systems:
  • Escherichia coli
  • Not provided