PDBe 5wkf

X-ray diffraction
2.95Å resolution

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA]
ATP + H(2)O = ADP + phosphate
NTP + H(2)O = NDP + phosphate
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m(7)G(5')pppR-RNA
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-11 alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 274 amino acids
Theoretical weight: 31.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13746 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Serine protease NS3 Chains: C, H
Molecule details ›
Chains: C, H
Length: 10 amino acids
Theoretical weight: 988 Da
Source organism: Dengue virus 1
Expression system: Not provided
UniProt:
  • Canonical: P17763 (Residues: 1608-1617; Coverage: 0%)
T-cell receptor alpha variable 30,T-cell receptor, sp3.4 alpha chain Chimera Chains: D, I
Molecule details ›
Chains: D, I
Length: 198 amino acids
Theoretical weight: 21.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
D30 TCR beta chain Chains: E, J
Molecule details ›
Chains: E, J
Length: 244 amino acids
Theoretical weight: 27.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 88.057Å b: 146.376Å c: 167.557Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.253
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided