PDBe 5wfo

X-ray diffraction
1.99Å resolution

Ligand-bound Ras:SOS:Ras complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase HRas, N-terminally processed Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 167 amino acids
Theoretical weight: 18.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Son of sevenless homolog 1 Chain: N
Molecule details ›
Chain: N
Length: 482 amino acids
Theoretical weight: 56.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07889 (Residues: 566-1046; Coverage: 36%)
Gene name: SOS1
Sequence domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I422
Unit cell:
a: 182.735Å b: 182.735Å c: 177.866Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.188
Expression system: Escherichia coli