PDBe 5wfc

X-ray diffraction
2.28Å resolution

Humanized mutant of the Chaetomium thermophilum Polycomb Repressive Complex 2 bound to the inhibitor GSK343

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Polycomb Protein EED Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 66.02 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: G0S8H7 (Residues: 1-565; Coverage: 89%)
Gene name: CTHT_0029920
Sequence domains: WD domain, G-beta repeat
Zinc finger domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 936 amino acids
Theoretical weight: 106.8 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: G0RYC6 (Residues: 530-691; Coverage: 22%)
  • Canonical: G0SDW4 (Residues: 191-950; Coverage: 50%)
  • nullnull
Gene names: CTHT_0006210, CTHT_0053230
Sequence domains:
Histone H3.1 Chain: D
Molecule details ›
Chain: D
Length: 11 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 23-33; Coverage: 8%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 117.587Å b: 137.794Å c: 222.332Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.203
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided