PDBe 5w9a

X-ray diffraction
2.74Å resolution

The structure of the Trim5alpha Bbox- coiled coil in complex LC3B

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tripartite motif-containing protein 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 23.04 KDa
Source organism: Macaca mulatta
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0PF16 (Residues: 95-287; Coverage: 39%)
Gene name: TRIM5
Microtubule-associated proteins 1A/1B light chain 3B Chains: C, D
Molecule details ›
Chains: C, D
Length: 114 amino acids
Theoretical weight: 13.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZQ8 (Residues: 4-117; Coverage: 91%)
Gene names: MAP1ALC3, MAP1LC3B

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 72.014Å b: 115.319Å c: 174.164Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.26 0.274
Expression system: Escherichia coli