PDBe 5w1f

X-ray diffraction
2.6Å resolution

Crystal structure of Ni(II)- and Ca(II)-bound human calprotectin

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein S100-A8 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 93 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05109 (Residues: 1-93; Coverage: 100%)
Gene names: CAGA, CFAG, MRP8, S100A8
Sequence domains: S-100/ICaBP type calcium binding domain
Protein S100-A9 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 114 amino acids
Theoretical weight: 13.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06702 (Residues: 1-114; Coverage: 100%)
Gene names: CAGB, CFAG, MRP14, S100A9
Sequence domains: S-100/ICaBP type calcium binding domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 57.02Å b: 77.611Å c: 222.722Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.262
Expression system: Escherichia coli BL21(DE3)