PDBe 5vzu

X-ray diffraction
2.7Å resolution

Crystal structure of the Skp1-FBXO31-cyclin D1 complex

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
S-phase kinase-associated protein 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 149 amino acids
Theoretical weight: 16.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63208 (Residues: 1-163; Coverage: 91%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
F-box only protein 31 Chains: B, D
Molecule details ›
Chains: B, D
Length: 488 amino acids
Theoretical weight: 55.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5XUX0 (Residues: 66-539; Coverage: 88%)
Gene names: FBX14, FBX31, FBXO31, PP2386
Sequence domains: F-box-like
G1/S-specific cyclin-D1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 17 amino acids
Theoretical weight: 1.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P24385 (Residues: 279-295; Coverage: 6%)
Gene names: BCL1, CCND1, PRAD1

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 87.679Å b: 156.235Å c: 155.522Å
α: 90° β: 104.21° γ: 90°
R-values:
R R work R free
0.183 0.18 0.233
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided