PDBe 5vo0

X-ray diffraction
3.9Å resolution

Structure of a TRAF6-Ubc13~Ub complex

Released:

Function and Biology Details

Reactions catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
TNF receptor-associated factor 6 Chains: A, D
Molecule details ›
Chains: A, D
Length: 172 amino acids
Theoretical weight: 19.77 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6IWL4 (Residues: 50-213; Coverage: 30%)
Gene names: si:dkey-56p7.3, traf6, zgc:63704
Sequence domains:
Ubiquitin-conjugating enzyme E2 N Chains: B, E
Molecule details ›
Chains: B, E
Length: 157 amino acids
Theoretical weight: 17.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61088 (Residues: 1-152; Coverage: 100%)
Gene names: BLU, UBE2N
Sequence domains: Ubiquitin-conjugating enzyme
Ubiquitin Chains: C, F
Molecule details ›
Chains: C, F
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P42212
Unit cell:
a: 181.114Å b: 181.114Å c: 97.414Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.252 0.299
Expression system: Escherichia coli BL21