› 5vcu

X-ray diffraction
1.85Å resolution

Crystal structure of ras-related c3 botulinum toxin substrate 1 isoform x2 from Naegleria fowleri in complex with GDP

Released:
DOI: 10.2210/pdb5vcu/pdb
PDB entry 5vcu coloured by chain, front view.
PDB entry 5vcu coloured by chain, side view.
PDB entry 5vcu coloured by chain, top view.
Source organism: Naegleria fowleri
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related c3 botulinum toxin substrate 1 isoform x2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 23.49 KDa
Source organism: Naegleria fowleri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1Z0YU85 (Residues: 1-200; Coverage: 100%)
Gene name: FDP41_011447
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P6522
Unit cell:
a: 48.38Å b: 48.38Å c: 624.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.211
Expression system: Escherichia coli BL21(DE3)

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