PDBe 5vc6

X-ray diffraction
2Å resolution

crystal structure of human WEE1 kinase domain in complex with PHA-848125

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Wee1-like protein kinase Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30291 (Residues: 291-575; Coverage: 44%)
Gene name: WEE1
Sequence domains: Protein kinase domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 50.43Å b: 44.92Å c: 64.8Å
α: 90° β: 102.91° γ: 90°
R-values:
R R work R free
0.22 0.22 0.264
Expression system: Escherichia coli BL21(DE3)