PDBe 5vbn

X-ray diffraction
2.35Å resolution

Crystal Structure of human DNA polymerase epsilon B-subunit in complex with C-terminal domain of catalytic subunit

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase epsilon subunit 2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 527 amino acids
Theoretical weight: 59.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P56282 (Residues: 1-527; Coverage: 100%)
Gene names: DPE2, POLE2
Sequence domains:
DNA polymerase epsilon catalytic subunit A Chains: B, F
Molecule details ›
Chains: B, F
Length: 145 amino acids
Theoretical weight: 16.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07864 (Residues: 2142-2286; Coverage: 6%)
Gene names: POLE, POLE1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 92.26Å b: 201.459Å c: 78.336Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.264
Expression system: Escherichia coli