Function and Biology Details
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Structure analysis Details
Length: 278 amino acids
Theoretical weight: 32.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
- Canonical: P10319 (Residues: 25-300; Coverage: 82%)
Ligands and Environments
No modified residues
Experiments and Validation Details
- Escherichia coli BL21(DE3)
- Not provided