PDBe 5v4b

X-ray diffraction
2.6Å resolution

Crystal structure of the Skp1-FBXW7-DISC1 complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero 12-mer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P63208 (Residues: 1-69, 82-163; Coverage: 89%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
F-box/WD repeat-containing protein 7 Chain: B
Molecule details ›
Chain: B
Length: 444 amino acids
Theoretical weight: 50.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q969H0 (Residues: 263-706; Coverage: 63%)
Gene names: FBW7, FBX30, FBXW7, SEL10
Sequence domains:
Disrupted in schizophrenia 1 protein Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW Q9NRI5 (Residues: 193-207; Coverage: 2%)
Gene names: DISC1, KIAA0457

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4122
Unit cell:
a: 233.514Å b: 233.514Å c: 108.438Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.219
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided