PDBe 5v1z

X-ray diffraction
2Å resolution

Crystal structure of the RPN13 PRU-RPN2 (932-953)-ubiquitin complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasomal ubiquitin receptor ADRM1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 13.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q16186 (Residues: 19-132; Coverage: 28%)
Gene names: ADRM1, GP110
Sequence domains: Proteasome complex subunit Rpn13 ubiquitin receptor
Ubiquitin Chains: C, D
Molecule details ›
Chains: C, D
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
26S proteasome non-ATPase regulatory subunit 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 22 amino acids
Theoretical weight: 2.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99460 (Residues: 932-953; Coverage: 2%)
Gene name: PSMD1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P31
Unit cell:
a: 100.852Å b: 100.852Å c: 37.571Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.146 0.182
Expression system: Escherichia coli BL21