PDBe 5v1y

X-ray diffraction
1.42Å resolution

Crystal structure of the ternary RPN13 PRU-RPN2 (940-953)-ubiquitin complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasomal ubiquitin receptor ADRM1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 13.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q16186 (Residues: 19-132; Coverage: 28%)
Gene names: ADRM1, GP110
Sequence domains: Proteasome complex subunit Rpn13 ubiquitin receptor
Ubiquitin Chains: C, D
Molecule details ›
Chains: C, D
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
26S proteasome non-ATPase regulatory subunit 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 16 amino acids
Theoretical weight: 1.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99460 (Residues: 940-953; Coverage: 2%)
Gene name: PSMD1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 36.129Å b: 96.098Å c: 57.605Å
α: 90° β: 96.38° γ: 90°
R-values:
R R work R free
0.141 0.14 0.175
Expression system: Escherichia coli BL21