PDBe 5uru

X-ray diffraction
2.41Å resolution

Insulin with proline analog DhP at position B28 in the R6 state

Released:
Source organism: Homo sapiens
Entry authors: Lieblich SA, Fang KY, Tirrell DA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: R3
Unit cell:
a: 79.259Å b: 79.259Å c: 79.874Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.187 0.246
Expression system: Escherichia coli