PDBe 5uq3

X-ray diffraction
3.6Å resolution

Crystal structure of human Cdk2-Spy1-P27 ternary complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-dependent kinase 2 Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 34.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P24941 (Residues: 1-298; Coverage: 100%)
Gene names: CDK2, CDKN2
Sequence domains: Protein kinase domain
Speedy protein A Chain: B
Molecule details ›
Chain: B
Length: 160 amino acids
Theoretical weight: 19.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5MJ70 (Residues: 61-213; Coverage: 49%)
Gene names: SPDY1, SPDYA, SPY1
Sequence domains: Cell cycle regulatory protein
Cyclin-dependent kinase inhibitor 1B Chain: C
Molecule details ›
Chain: C
Length: 201 amino acids
Theoretical weight: 22.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46527 (Residues: 1-198; Coverage: 100%)
Gene names: CDKN1B, KIP1
Sequence domains: Cyclin-dependent kinase inhibitor

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3121
Unit cell:
a: 124.65Å b: 124.65Å c: 88.49Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.243 0.324
Expression system: Escherichia coli