PDBe 5ulo

X-ray diffraction
2.14Å resolution

Crystal Structure of 14-3-3 zeta in Complex with a Serine 124-phosphorylated TBC1D7 peptide

Released:
Source organism: Homo sapiens
Entry authors: DONG A, HU J, MADIGAN J, WALKER JR, Bountra C, Arrowsmith CH, Edwards AM, TONG Y, Structural Genomics Consortium (SGC)

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
TBC1 domain family member 7 Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9P0N9 (Residues: 115-126; Coverage: 4%)
Gene names: HSPC239, TBC1D7, TBC7

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 69.709Å b: 71.575Å c: 129.899Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.27
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided