PDBe 5udh

X-ray diffraction
3.24Å resolution

HHARI/ARIH1-UBCH7~Ubiquitin

Released:

Function and Biology Details

Reactions catalysed:
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
E3 ubiquitin-protein ligase ARIH1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 470 amino acids
Theoretical weight: 55.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: NEW Q9Y4X5 (Residues: 90-557; Coverage: 84%)
Gene names: ARI, ARIH1, HUSSY-27, MOP6, UBCH7BP
Sequence domains: IBR domain, a half RING-finger domain
Ubiquitin-conjugating enzyme E2 L3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 156 amino acids
Theoretical weight: 18.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: NEW P68036 (Residues: 1-154; Coverage: 100%)
Gene names: UBCE7, UBCH7, UBE2L3
Sequence domains: Ubiquitin-conjugating enzyme
Ubiquitin Chain: E
Molecule details ›
Chain: E
Length: 82 amino acids
Theoretical weight: 9.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: NEW P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 184.571Å b: 76.788Å c: 147.724Å
α: 90° β: 107.33° γ: 90°
R-values:
R R work R free
0.225 0.222 0.279
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4