› 5u23

X-ray diffraction
1.45Å resolution

X-ray structure of the WlaRG aminotransferase from Campylobacter jejuni in complex with TDP-Qui3N

Released:
DOI: 10.2210/pdb5u23/pdb
PDB entry 5u23 coloured by chain, front view.
PDB entry 5u23 coloured by chain, side view.
PDB entry 5u23 coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Structural investigation on WlaRG from Campylobacter jejuni: A sugar aminotransferase.
Dow GT, Gilbert M, Thoden JB, Holden HM
Protein Sci 26 586-599 (2017)
PMID: 28028852

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189586 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative aminotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 382 amino acids
Theoretical weight: 44.04 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ALS9 (Residues: 1-360; Coverage: 100%)
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NA 2 x NA
Ligand TQP 4 x TQP
Ligand EDO 7 x EDO
Ligand CL 1 x CL
Ligand MPO 1 x MPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 107.824Å b: 56.644Å c: 124.549Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.129 0.127 0.153
Expression system: Escherichia coli

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Citations

6 citation in other articles

EpsN from Bacillus subtilis 168 has UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase activity in vitro.
Kaundinya et al. (2018)
5 more

1 mention without citation

Structural investigation on WlaRG from Campylobacter jejuni: A sugar aminotransferase.
Dow et al. (2017)