› 5tyt

X-ray diffraction
2.4Å resolution

Crystal Structure of the PDZ domain of RhoGEF bound to CXCR2 C-terminal peptide

Released:
DOI: 10.2210/pdb5tyt/pdb
PDB entry 5tyt coloured by chain, front view.
PDB entry 5tyt coloured by chain, side view.
PDB entry 5tyt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of PDZ-mediated chemokine receptor CXCR2 scaffolding by guanine nucleotide exchange factor PDZ-RhoGEF.
Spellmon N, Holcomb J, Niu A, Choudhary V, Sun X, Zhang Y, Wan J, Doughan M, Hayden S, Hachem F, Brunzelle J, Li C, Yang Z
Biochem Biophys Res Commun 485 529-534 (2017)
PMID: 28179147

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor 11 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 89 amino acids
Theoretical weight: 9.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15085 (Residues: 40-126; Coverage: 6%)
Gene names: ARHGEF11, KIAA0380
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 61.739Å b: 66.633Å c: 168.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure function relations in PDZ-domain-containing proteins: Implications for protein networks in cellular signalling.
Manjunath et al. (2018)
1 more

2 mentions without citation

Therapeutic inhibition of CXCR1/2: where do we stand?
Sitaru et al. (2023)
1 more