PDBe 5tuz

X-ray diffraction
1.95Å resolution

Structure of human GLP SET-domain (EHMT1) in complex with inhibitor MS0124

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase EHMT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 30.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H9B1 (Residues: 1006-1266; Coverage: 20%)
Gene names: EHMT1, EUHMTASE1, GLP, KIAA1876, KMT1D
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 74.94Å b: 95.78Å c: 102.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.201
Expression system: Escherichia coli BL21(DE3)