› 5ttj

X-ray diffraction
2.2Å resolution

Crystal Structure of Nicotine Oxidoreductase from Pseudomonas putida

Released:
DOI: 10.2210/pdb5ttj/pdb
PDB entry 5ttj coloured by chain, front view.
PDB entry 5ttj coloured by chain, side view.
PDB entry 5ttj coloured by chain, top view.
Source organism: Pseudomonas putida S16
Primary publication:
Structural Analysis Provides Mechanistic Insight into Nicotine Oxidoreductase from Pseudomonas putida.
Tararina MA, Janda KD, Allen KN
Biochemistry 55 6595-6598 (2016)
PMID: 27933790

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotine dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 490 amino acids
Theoretical weight: 53.67 KDa
Source organism: Pseudomonas putida S16
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: F8G0P2 (Residues: 1-482; Coverage: 100%)
Gene names: PPS_4081, nicA2
Sequence domains: Flavin containing amine oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

1 bound ligand:
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 67.973Å b: 61.564Å c: 92.277Å
α: 90° β: 92.51° γ: 90°
R-values:
R R work R free
0.176 0.175 0.231
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

X-Ray Crystallography in Structure-Function Characterization of Therapeutic Enzymes.
Papageorgiou AC. (2019)
10 other articles

3 mentions without citation

The enzymes of microbial nicotine metabolism.
Fitzpatrick PF. (2018)
2 more